含血栓的曲张静脉血管壁T和B淋巴细胞浸润的定量研究

Research of distribution and quantitation of T and B lymphocytes infiltration in vascular wall of varicose veins with thrombus

目的:探讨含血栓的曲张大隐静脉血管壁T、B淋巴细胞的浸润情况。方法:收集单纯曲张大隐静脉管壁标本11例,含血栓的曲张大隐静脉管壁标本11例,另设8例正常大隐静脉管壁标本作对照,行免疫组织化学染色,观察血管壁中T、B淋巴细胞的分布。结果:血栓组管壁各层中T、B淋巴细胞呈现"聚集性"增多;曲张组在滋养血管周围可见少量淋巴细胞浸润。血栓组T、B淋巴细胞计数与曲张组、对照组差异均有统计学意义(P<0.01),曲张组与对照组之间差异无统计学意义(P>0.05)。结论:静脉血栓形成可诱发静脉血管壁T、B淋巴细胞的重塑。

Objective：To investigate the infiltrating changes of T and B lymphocytes in vascular wall of great saphenous varicose veins with thrombus.Methods：Collecting 11 specimens of simple varicose veins,Eleven specimens of varicose veins with thrombus and 8 specimens of normal venous tissue used as control.Detected the distribution of T and B lymphocytes by immunohistochemical staining.Results：Increased T and B lymphocytes infiltration was ＂gathered＂ in intima,media and adventitia of vascularwall in thrombus group.Varicose group can be seen a few T,B lymphocytes scattered around vasa vasorums.The difference of T and B lymphocytes counting among thrombus group,varicose group,control group was significantly （P0.01）,there was no significant difference between varicose and control group （P0.05）.Conclusion：T and B lymphocytes remodeling in the vascularwall can be induced by venous thrombus.