无花果叶水溶性成分的提取及其急性毒性试验

PREPARATION AND ACUTE TOXICITY OF WATER SOLUBLE COMPONENT FROM FIG LEAF

报道了无花果叶中水溶性成分的提取及其急性毒性试验。取干燥的无花果３００ｇ，用８９００ｇ蒸馏水分４次重复提取，得提取液７７３９ｇ，浓缩后得到浓缩液２８０ｇ，经测定其相对密度（ｄ）为１．１４３，质量浓度为３２４．６ｇ／Ｌ，干态物质总产量为７９．５ｇ，提取率达３１．０％。用灌胃法（ｉｇ）、腹腔注射法（ｉｐ）测试了小鼠对水提取物的急性毒性反应。结果表明，ｉｇ试验２５倍临床口服剂量以下未发现毒性反应，ｉｐ试验ＬＤ５０＝１．０４３９ｇ／ｋｇ，相当于临床口服剂量的３．３倍，Ｌ９５＝０．８９４６～１．

The preparation and acute toxicity of water soluble component from fig leaf was reported. Dried fig leaf 300 g was extracted four times with distilled water 8900 g under reflux. Extract liquor 7739 g was collected and concentrated subsequently in vaccum. The concentrate 280 g was acquired, which possesses relative density 1.143, mass concentration 324.6 g/L. Total yield of dry matter from the concentrate is 79.5 g, the yield of dry matter per unit weight of the leaf reaches 31.0%. Acute toxicity was tested with ig and ip in rat. As a result, no toxicity effect was observed in ig below 25 times clinical oral dosage, LD50=1.0439 g/kg in ip, which is equivalent to 3.3 times clinical oral dosage, L95=0.89461.2033 g/kg.