白花丹参上调Bcl-2抑制内皮细胞凋亡

Salvia miltiorrhiza bge.f.alba up-regulates Bcl-2 and inhibits apoptosis of human umbilical vein endothelial cells

目的研究白花丹参对过氧化氢(H2O2)诱导的人脐静脉血管内皮细胞(HUVEC)凋亡的抑制作用及对凋亡相关基因Bcl-2表达的调节。方法应用酶消化灌注法分离人脐静脉血管内皮细胞,采用形态学观察和Ⅷ因子抗体免疫荧光检测法进行鉴定。取对数生长期的细胞分组进行干预(白花丹参高剂量组0.10g/ml,白花丹参低剂量组0.01g/ml),应用流式细胞技术观察细胞凋亡情况,应用免疫细胞荧光技术检测凋亡相关基因Bcl-2的表达。结果在白花丹参干预下,H2O2诱导的HUVEC凋亡率降低(高剂量组P<0.01,低剂量组P<0.05),高剂量组Bcl-2的表达增加(P<0.01)。结论白花丹参对H2O2诱导的HUVEC凋亡具有抑制作用,这种作用与Bcl-2表达上调有关。

Objective To study the influence of Salvia miltiorrhiza bge.f.alba（SMA） on apoptosis and Bcl-2 expression in human umbilical vein endothelial cell（HUVEC） in vitro.Methods HUVECs were isolated using perfusion and enzyme digestion methods,and the obtained cells were identified by morphological observation and Ⅷ：Ag immunoreactivity examination.The cells in the exponential phase of growth were treated with H2O2 and different concentrations of SMA（high dose：0.10 g/ml,low dose：0.01 g/ml）.The cell apoptosis was determined by flow cytometric analysis and Bcl-2 expression was examined by immunofluorescence mehtod.Results SMA significantly decreased the H2O2-induced apoptosis of HUVECs（P 〈 0.01 in high dose group and P 〈 0.05 in low dose group）,and significantly increased the expression of Bcl-2 in high dose group（P 〈 0.01）.Conclusion SMA can inhibit H2O2-induced apoptosis of HUVEC,which might be associated with the increase of Bcl-2 expression.