rhIL-1α/rhTGF-β1诱导下大鼠髁突软骨细胞PRG4的表达

Effects of IL-1 alpha/TGF-beta1 on the PRG4 expression in the chondrocytes derived from rat condyle cartilage

目的:观察rhTGF-β1对rhIL-1α作用下大鼠髁突软骨细胞蛋白多糖4(PRG4)表达的影响。方法:酶消化法获取髁突软骨细胞,甲苯胺蓝染色鉴定。细胞上清液中加入10ng/ml的rhIL-1α,48h后加入10ng/ml的rhTGF-β1。不同时间点应用RT-PCR检测PRG4mRNA的表达情况,ELISA法检测PRG4蛋白的分泌变化。结果:加入rhIL-1α24、48、72h组的PRG4表达量与对照组均有统计学差异(P<0.05),且逐渐降低。48h组再加入rhTGF-β124h后PRG4表达量与对照组无统计学差异(P>0.05),而48h后与各组均有统计学差异(P<0.05),且最高。结论:rhTGF-β1可以上调髁突软骨细胞PRG4的表达,且可逆转IL-1α的下调作用。

Objective：To investigate the effects of interleukin-1（IL-1α）and transforming growth factor-β1（TGF-β1）on the expression and secretion of proteoglycan 4（PRG4）by the articular chondrocytes derived from rat condyle.Methods：The chondrocytes were isolated from rat mandibular condyle by enzyme digestion and maintained in a monolayer culture system.After exposure to rhIL-1α（10 ng/ml）and rhTGF-β1（10 ng/ml）alone or in combination for indicated time course,the PRG4 mRNA expression and secretion by chondrocytes were assessed by RT-PCR and ELISA respectively.Results：Exposure of chondrocytes to IL-1α resulted in a pronounced reduction in the expression and abundance of secreted PRG4 in a time-dependent manner（P〈0.05）.Moreover,following IL-1α pretreatment for 48 h,TGF-β1 could restore the expression of PRG4 to a higher level（P〈0.05）48 h after treatment.Conclusion：Condylar cartilage production of PRG4 is positively regulated by TGF-β1 and negatively by IL-1α.Furthermore,TGF-β1 is able to antagonize the inhibitory effects of IL-1α on the PRG4 expression in cultured condylar chondrocytes.