摘要
普通水绵〔Ｓｐｉｒｏｇｙｒａｃｏｍｍｕｎｉｓ（Ｈａｓｓａｌ）Ｋüｔｚｉｎｇ〕的超氧物歧化酶（ＳＯＤ）含有２条锰型和１条铁型同工酶主带。低浓度的Ｚａｒｏｕｋ培养液对ＳＯＤ影响不明显，浓度提高到３倍以上时造成渗透胁迫，在一定时间内，ＳＯＤ比活升高，但培养时间继续延长则下降。在百草枯１ｍｍｏｌ／Ｌ和２ｍｍｏｌ／Ｌ培养６ｄ和４ｄ时，普通水绵ＳＯＤ比活最高，此后则下降；２ｍｍｏｌ／Ｌ组的ＭＤＡ含量在试验期内一直缓缓上升。５倍的Ｚａｒｏｕｋ培养液和２ｍｍｏｌ／Ｌ百草枯进行培养后，ＦｅＳＯＤ带消失，ＭｎＳＯＤ主活性带有增强的趋势。
The effects of osmatic stress and paraquat on
SOD in Spirogyra communis (Hassall) Kützing are reported. Different concentration of Zarrouk
culture medium and culture time or paraquat treatments have different effects on SOD specific
activity in Spirogyra communis ; no obvious effect was appeared by low concentration and
short time, but as concentration increased to 3 times, osmatic stress will be occured. SOD
specific activities first increased and decreased afterwards; MDA content always increased as
time prolonged. SOD isozymograms show the presence of Fe SOD and Mn SOD bands in
Spirogyra communis , but osmatic and chemical stress have different effects on different type of
SOD isozymes. If Spirogyra communis is cultured in 5 times concentration of Zarrouk culture
medium or 2 mmol/L paraquat, Fe SOD band will disappear and Mn SOD bands will enhanced.
出处
《植物资源与环境》
CAS
CSCD
1999年第2期13-17,共5页
Journal of Plant Resources and Environment
基金
江苏省自然科学基金
