支气管哮喘患者血清中8-异前列腺素水平研究

Levels of 8-Isoprostane in Serum of Patients with Bronchial Asthma

目的探讨支气管哮喘患者血清中8-异前列腺素(8-iso-PG)水平检测的临床意义。方法选取2009年2月至8月就诊于昆明医学院第二附属医院呼吸内科的18例哮喘急性发作期患者为哮喘组,经治疗后达缓解期。选取2009年8月来自昆明医学院第二附属医院的职工体检志愿者20例为健康对照组。测定所有研究对象血清中8-iso-PG水平及肺功能指标FEV1%pred。结果哮喘患者急性发作期血清中8-iso-PG水平均高于缓解期和健康对照组[(157.46±46.99)pg/mL比(43.52±13.62)pg/mL和(15.23±1.96)pg/mL,P<0.01],缓解期哮喘患者血清中8-iso-PG水平也高于健康对照组(P<0.01)。哮喘患者急性发作期血清中8-iso-PG水平与FEV1%pred呈负相关(r=-0.533,P<0.05)。结论 8-iso-PG作为氧化应激反应的标记物参与了哮喘的发病,通过监测其在哮喘患者血清中的水平可以反映哮喘患者体内氧化应激反应状态,可以作为一种评估哮喘患者病情严重程度及随访治疗效果的指标。

Objective To investigate levels of 8-isoprostane in serum of patients with bronchial asthma.Methods Eighteen patients diagnosed with acute exacerbation of asthma were enrolled as the experimental group from Department of Respiratory Medicine from February 2009 to August 2009.After treatment all the patients reached remission.Twenty healthy workers from Department of Respiratory Medicine were enrolled as the control group in August 2009.The levels of 8-isoprostane in serum of all subjects were measured,and their FEV1%pred was also evaluated.Results The levels of 8-isoprostane in serum were significantly higher in patients with acute exacerbation of asthma compared with those in remission stage and the healthy control group [（157.46±46.99）pg/mL vs.（43.52±13.62）pg/mL and（15.23±1.96）pg/mL,P0.01].Meanwhile the levels of 8-isoprostane in serum of patients with asthma in remission stage were significantly higher compared with the healthy control group（P0.05）.The levels of 8-isoprostane in serum were negatively correlated with FEV1%pred in the asthma group（r=-0.533,P0.05）.Conclusions 8-isoprostane as a marker of oxidative stress response involves in the pathogenesis of asthma.Monitoring 8-isoprostane levels in serum may reflect the state of oxidative stress,and may be useful for severity judgment and follow-up of treatment effectiveness in patients with asthma.