低分子肝素对高脂饮食大鼠非酒精性脂肪性肝炎模型的影响

Low molecular weight heparin attenuating non-alcoholic steatohepatitis caused by high-fat diet in the rat

目的观察低分子量肝素对长期摄入高脂饮食所致大鼠非酒精性脂肪性肝炎的干预作用,探讨其机制。方法雄性SD大鼠40只,随机分为正常对照组(n=10)及模型组(n=10),模型组喂以高脂饮食共8周;其余20只在高脂饮食8周后分为低分子肝素低剂量治疗组(n=10),在继续高脂饲料喂养的基础上,加用低分子量肝素治疗[50IU/(kg·d)];低分子肝素高剂量治疗组(n=10),在继续高脂饲料喂养基础上,加用低分子量肝素治疗[200IU/(kg·d)],共治疗2周。结果低分子肝素高剂量组[200IU/(kg·d)]在抑制肝脏慢性炎症、防止肝脏脂质堆积方面较模型组有统计学差异,而低剂量组[50IU/(kg·d)]作用不如高剂量组作用明显。结论低分子量肝素能够抑制肝脏慢性炎症的发生,调节脂质在肝脏中的堆积,有治疗非酒精性脂肪性肝炎的作用,且其作用效果与剂量相关。其作用机制可能是调节血脂,降低血清中TNF-α的水平。

Objective To investigated interference of low molecular weight heparin（ LMWH） on non-alcoholic steatohepatitis caused by high-fat diet in the rat and study its mechanism. Methods M ale Sprague-Dawley rats（ n = 40） were randomly divided into the control group（ n = 10） and the model group（ n = 10） ： the model group was given a high-fat diet for 8 weeks; the low dosage treatment group（ n = 10） and high dosage treatment group（ n = 10） ： one received low doses of LM WH [50 IU /（ kg·d） ]and the other received 200 IU /（ kg·d） for 2 weeks,after 8 weeks of high-fat diet. Results Compared with the model group,a high dose of LMWH[200 IU /（ kg. d） ] significantly inhibited chronic inflammation and attenuated lipid deposits in the liver,while a low dose of LM WH was not significant. Conclusion LM WH has an excellent therapeutic effect on non-alcoholic fatty liver disease （ NAFLD） by inhibiting chronic inflammation and attenuating lipid deposits in the liver,which is closely related to the dosage. Its mechanism may be that LMWH regulates the level of blood fat and decreases the level of TNF-α in the serum.