乳酸杆菌代谢物RNA组分对小鼠免疫细胞功能影响的研究

Effects of Lactobacilli metabolite RNA on the immune function in mice

目的研究乳酸杆菌代谢物RNA组分对免疫细胞功能的影响,为进一步研究其可能存在的生物学功能打基础。方法采用细胞培养技术、Western Blot、ELISA和MTT等方法。结果乳酸杆菌代谢物RNA组分对全脾细胞和脾脏来源的DC增殖有促进作用,对骨髓来源的DC有抑制作用,它不刺激巨噬细胞数量增殖,但可以明显增强巨噬细胞的吞噬功能。用乳酸杆菌代谢物RNA组分冲激脾脏来源的DC可以轻微增强其杀伤YAC-1的作用和显著促进INF-γ分泌,但相同条件下对BMDC杀伤YAC-1的作用是抑制的,能抑制BMDC分泌INF-γ,但可以增加BMDC刺激全脾细胞的增殖和NF-κB的表达上调。结论乳酸杆菌代谢物RNA组分对小鼠免疫细胞功能具有调节作用。

Objective To study the effect of Lactobacilli metabolite RNA on the immune function. Method The effect of Lactobacilli metabolite RNA was measured with MTT, Western Blot and ELISA. Result Lactobacilli metabolite RNA promoted the proliferation of spleen cells and spleen-derived DCs, whereas it inhibited proliferation of BMDC. It showed no effect on the proliferation of macmphage but remarkably enhanced the phageeytosis of macrophage; it facilitated the cytotoxicity of DCs against YAC-1 cells and secretion of INF-γ; while under the same condition, it suppressed the cytotoxicity against YAC-1 cells by BMDC and inhibited the secretion of INF-γ, by BMDC, compared with the control group. But BMDC treated with Lactobacilli metabolite RNA had promoted the proliferation of spleen cells and up-regulated the expression of NF-κB on BMDC. Conclusion Lactobacilli metabolite RNA can regulate the function of immunocyte in mice.