转化生长因子-β1基因多态性与冠心病的相关性研究

Association of polymorphisms of transforming growth factor-beta 1 gene with coronary heart disease

目的探讨转化生长因子-β1（TGF-β1）基因多态性与冠心病的相关性。方法采用聚和酶链反应一限制性片断长度多态性（PCR—RFLP）技术，检测300例冠心病患者（冠心病组）及300例健康人（对照组）-509C／T位点的TGF-β1的基因多态性，同时检测血清血脂和超敏C反应蛋白（hsCRP）水平。结果冠心病组血清超敏C反应蛋白、总胆固醇、低密度脂蛋白胆固醇、载脂蛋白B水平和C、T等位基因频率明显大于对照组，差异有统计学意义（P〈0．01，P〈0．05）。结论TGF-β1基因-509C／T基因多态性与冠心病的发病有相关性；C等位基因可能是冠心病发病的易感基因。

Objective To explore the association of transforming growth factor-beta 1 （ TGF-β1 ） polymorphisms with coronary heart disease（CHD）. Methods The 509C/T polymorphisms of TGF-β1 gene were analyzed by polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） in 300 patients of CHD and 300 healthy individuals, the levels of serum lipids and hsCRP were also studied. Results The levels of hsCRP,TC ,LDL, ApoB in serum and the frequencies of C,T allele at position-509 of TGF-β1 gene were statistically significant higher than the controls （ P 〈 0. 01, P 〈 0. 05 ）. Conclusion The polymorphisms of TGF^I-509C/T were associated with CHD. C allele should be the susceptibility gene in the occurence of CHD.