低剂量三氯乙烯诱导下谷胱甘肽转移酶Ω-1的表达变化

Expression changes of glutathione transferase omega 1 induced by low dose trichloroethylene

目的分析低剂量三氯乙烯(TCE)诱导的细胞兴奋效应时,谷胱甘肽转移酶Ω-1(GSTO-1)的表达变化。方法利用前期的双向电泳和质谱结果,参照前期兴奋效应的剂量设计,提取染毒细胞的mRNA和总蛋白,进一步使用实时定量RT-PCR和Western blotting分别从mRNA和蛋白水平上验证GSTO-1的表达变化。结果 Western blotting分析发现,0.1mmol/LTCE剂量组能诱导GSTO-1蛋白表达是对照组的2倍,但随着剂量的增加,蛋白表达改变不明显(与对照组相比,P<0.05)。RT-PCR结果提示:低剂量TCE能诱导GSTO-1基因表达增加,随着剂量的增加,表达反而有所减少。结论低剂量TCE能诱导GSTO-1基因及蛋白表达增加。

Objective To determine the expression of glutathione transferase omega 1（GSTO-1） during hormesis induced by low dose trichloroethylene（TCE）.Methods Based on the dose-response of hormesis induced by TCE,the total protein and mRNA from four groups was extracted,and expression of GSTO-1 was detected by western blotting and RT-PCR technology.Results The study showed that increases of GSTO-1 protein expression were induced by TCE at low dose（0.1mmol /L） TCE,but there was no significant different between the high-dose group and the control（P 0.05）.RT-PCR analysis presented the expression of GSTO-1 gene in low-dose group was increased,but the opposite effect was observed in the high-dose group.Conclusion GSTO-1 may be play a key role in hormesis induced by TCE.