摘要
将从处于一定成熟度的香蕉果肉中纯化出的mRNA反转录成cDNA。cDNA纯化后与EcoRI转接头相连，并插入λgt10载体，经体外包装，侵染大肠杆菌C600hfl菌株，从而构建了香蕉果实的cDNA文库。
Poly(A)+ mRNA from pulps of ripening banana was purified and reverse-transcribed to cDNA. The purified cDNA was ligated with EcoRI adaptor and. inserted intoλgt10 vector. The ligated DNA was packed into in vitro packaging system. The packaging mix was infected with E.coli C600hfl and the cDNA library from banana fruitwas thus constructed.
出处
《热带作物学报》
CSCD
1999年第1期25-28,共4页
Chinese Journal of Tropical Crops
基金
国家自然科学基金
