FADD蛋白在急性肝衰竭小鼠肝细胞中的表达

Expression of FADD protein in hepatocyte of mice with acute hepatic failure

目的探讨解毒化瘀颗粒对抑制急性肝衰竭小鼠肝细胞凋亡及凋亡蛋白FADD的影响,进而揭示其治疗肝衰竭的机制。方法将昆明小鼠随机分为空白组、模型组、解毒化瘀颗粒组、安宫牛黄丸组、乳果糖组,用D-GalN+LPS腹腔注射构建急性肝衰竭的小鼠模型,观察各组小鼠存活率;应用原位末端转移酶标记技术(TUNEL)检测肝细胞凋亡的情况;应用免疫组化法和图像分析技术检测肝细胞FADD蛋白的表达。结果成模后48 h存活率解毒化瘀颗粒组高于其他药物组;TUNEL法检测结果显示:解毒化瘀颗粒组肝细胞凋亡指数明显减少,与其余药物干预组及模型组比较均有显著性差异(P<0.01或0.05);免疫组织化学染色结果显示:促凋亡因子FADD蛋白在解毒化瘀颗粒组肝组织中表达量低,而在其他药物干预组及模型组中则呈现高表达,有显著性差异(P<0.01或0.05)。结论解毒化瘀颗粒可通过下调内毒素肝衰竭模型中肝细胞FADD蛋白表达,并发挥其抑制凋亡效应,进而拮抗肝衰竭。

Objective It is to approach the influence of Jiedu Huayu Keli inhibiting hepatocyte apoptosis and apoptosis protein FADD in acute hepatic failure mice and then to reveal the mechanism of Jiedu Huayu Keli on hepatic failure.Methods Kunming mice were randomly divided into blank group,model group,Jiedu Huayu Keli group,Angong Niuhuang Wan group and lactulose group.The mice models with acute hepatic failure were induced with D-GalN and LPS intraperitoneal injection.The survival rates of the mice in all the groups were observed.Hepatocyte apoptosis was detected with TUNEL.The expression of hepatocyte FADD protein was detected with immunohistochemisty and image analysis.Results The survival rate after model established for 48 h in Jiedu Huayu Keli group was higher than that in other drugs groups.TUNEL detected results showed that the index of hepatocyte apoptosis in Jiedu Huayu Keli group was obviously decreased and there were all significant differences compared with other drugs groups and model group（P0.01 or 0.05）.Immunohistochemisty staining results showed that the expression of FADD protein in Jiedu Huayu Keli group was low and that in other drugs groups and model group were high and there were all significant differences（P0.01 or 0.05）.Conclusion Jiedu Huayu Keli can treat hepatic failure through down regulating the expression of hepatocyte FADD protein in endotoxin hepatic failure model to develop its inhibiting apoptosis effect.