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芹菜素抑制卵巢癌细胞株SKOV3生长的作用研究 被引量：5

Inhibitory effects of apigenin on human ovarian cancer cell strain SKOV3 growth
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摘要 目的探讨芹菜素对人卵巢癌细胞株SKOV3细胞增殖的抑制作用。方法运用细胞培养、光镜观察及四甲基偶氮唑蓝(MTT)比色法检测芹菜素对SKOV3细胞增殖的抑制作用,流式细胞仪技术分析芹菜素对SKOV3细胞增殖周期的影响。结果 10～80μmol/L芹菜素均能抑制SKOV3细胞的生长,且呈明显的时间、剂量依赖关系。细胞周期分析显示芹菜素组G2/M期细胞比例明显增多,呈量效关系。结论芹菜素抑制SKOV3细胞增殖,可能通过使SKOV3细胞停滞在G2/M期而诱导其凋亡。 Objective It is to approach the inhibitory effects of apigenin on human ovarian cancer cell strain SKOV3 cells proliferation.Methods The inhibitory effects of apigenin on SKOV3 cells proliferation were detected with cell culture,observation with light microscope and MTT colorimetric method.The influence of apigenin on SKOV3 cells proliferation cycle was analyzed with flow cytometry.Results 10～80 μmol/L apigenin could all inhibit the growth of SKOV3 cells,which was showed the time and dose dependent relationship.Cell cycle analysis showed that the ratio of G2/M period cells was obviously increased,which was showed the dose dependent relationship.Conclusion Apigenin can inhibit the proliferation of SKOV3 cells and might induce the apoptosis through making the cell cycle stagnated at G2/M period.
作者 任秀聪 张宏煜 李彦敏
机构地区 河北省石家庄市第一医院
出处 《现代中西医结合杂志》 CAS 2010年第35期4532-4534,共3页 Modern Journal of Integrated Traditional Chinese and Western Medicine
关键词 芹菜素 卵巢癌细胞株 SKOV3 apigenin ovarian cancer cell strain SKOV3
分类号 R-322 [医药卫生]
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参考文献4

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二级参考文献26

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共引文献82

同被引文献74

  • 1任环宇,唐修文.芹菜素对肺癌细胞的抗增殖作用和抗肿瘤药物的增敏作用[J].浙江大学学报（医学版）,2011,40(5):508-514. 被引量：14
  • 2张胜华,粟俭,甄永苏.丹酚酸A抑制核苷转运并增强化疗药物的抗肿瘤作用[J].药学学报,2004,39(7):496-499. 被引量：15
  • 3金雪瑛,任常山.芹菜素抑制人乳腺癌MDA-MB-231细胞血管内皮生长因子表达[J].中国现代医学杂志,2007,17(4):402-405. 被引量：8
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引证文献5

二级引证文献23

现代中西医结合杂志
2010年 第35期

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