绿色木霉菌产黄色素液态发酵条件的优化

Optimization of fermentation conditions in liquid state of Trichoderma viride to produce yellow pigment

以绿色木霉菌(Trichoderm aviride)为试验菌株,在培养基碳源、氮源组成,装液量,摇床转速,接种量,培养时间及培养温度等单因素试验的基础上,采用Box-Behnken设计及响应面分析法优化T.viride产天然黄色素的培养基组成和发酵工艺条件。结果表明,在试验范围内,T.viride产黄色素的优化培养条件为:马铃薯液态培养基(250g.L-1马铃薯浸汁,2g.L-1Na2HPO4),外加碳源选择21.7g.L-1木糖,摇瓶装液量为50ml.(250ml)-1,培养温度35℃,接种量6%(体积),摇床转速120r.min-1,培养时间152h。在优化培养条件下发酵液黄色素的色价为(52.8±0.8)U.ml-1。马铃薯培养基中添加尿素、硫酸铵、蛋白胨、牛肉浸粉等氮源会抑制黄色素的生成。

The composition of nutrient medium and the condition of fermentation process were optimized for T.viride producing yellow pigment by three-factor Box-Behnken design and response surface analysis,which was based on the experimental results of single factor for testing bacterial strain Trichoderma viride,including the source of carbon and nitrogen,liquid loading,rotation speed,inoculum concentration,culture time and temperature etc.The results show that the optimum culture medium components and conditions are：liquid culture medium based on PDA（250 g.L-1 potato extract and 2 g.L-1 Na2HPO4）,35℃,inoculum 6%（vol）,carbon source 21.7 g.L-1 xylose,loading liquid amount 50 ml in 250 ml Erlenmeyer flask and culture time 152 h at 120 r.min-1.In the optimal conditions,the color value of yellow pigment obtained is（52.8±0.8）U.ml-1.The formation of yellow pigment would be inhibited by adding urea,ammonium sulfate,peptone,beef extract powder as the source of nitrogen.