尿样中三种蛋白质的毛细管电泳分离检测方法研究

Determination of three proteins in urine by capillary electrophoresis

目的:建立毛细管电泳分离测定人尿样中转铁蛋白、白蛋白和血红蛋白的新方法。方法:通过选择运行缓冲溶液种类及浓度、pH、表面活性剂种类及浓度、分离电压、进样时间对蛋白质分离效果的影响,优化了毛细管电泳法分离转铁蛋白、白蛋白和血红蛋白的条件。结果:利用此方法测定三种蛋白质的含量,浓度在0.01到1.00gL-1范围内与峰电流呈良好的线性关系,检出限均为10-4gL-1。结论:所建立的方法用于人尿样中转铁蛋白、白蛋白和血红蛋白的测定,结果满意。

Objective：A method has been developed for the separation and determination of transferrin,albumin and hemoglobin in human urine by capillary electrophoresis.Methods：Effects of separation conditions such as running buffer type and concentration,pH,surfactant types and concentration,voltage,sampling time on the separation of transferrin,albumin and hemoglobin have been investigated and optimized in detail.Results：The protocol has been charactrized with cyclic voltammogram and potentiostatic method.A good linear relationship between peak current and the three kinds of proteins concentration of 0.01～1.00 g L^-1 was obtained,and the detection limits were 10^-4 g L^-1.Conclusions：The presented procedure has been successfully applied for the determination of transferrin,albumin and hemoglobin in human urine by capillary electrophoresis with satisfactory results.