摘要
Objective: The aim of our study was to investigate the expressions of Survivin and Caspase-3 in the hepatocellular carcinoma (HCC) and the paracancerous liver tissues, and define the relationship between the expression and the patients survival rate after surgery. Methods: The expressions of PCNA, Survivin and Caspase-3 were detected by immunohistochemistry. PCNA positive level was used to account proliferating cell nuclear antigen proliferating index (PCNA-PI). The apoptosis index of HCC tissues was detected by TUNEL. Results: The expression of Survivin in HCC tissue was higher than that in paracancerous tissue (P < 0.05), while it was related with portal vein thrombosis, tumor size and histological differentia- tion (P < 0.05). In HCC tissues, the PCNA-PI of the Survivin positive group was higher than the negative (P < 0.05) group, and the AI of the Survivin positive group was lower than the negative group (P < 0.05). The expression of Caspasse-3 in HCC tissue was lower than that in paracancerous tissue (P < 0.05), while it was related with HBsAg and histological differentiation (P < 0.05). In HCC tissues, the average PCNA-PI of the Caspase-3 positive group was lower than that of the negative group (P < 0.05), and the average AI of the Caspase-3 positive group was higher than that of the negative group (P < 0.05). There was negative correlation between the expressions of Survivin and Caspase-3 in HCC (r = -0.242, P < 0.05). The survival rate of the Survivin positive group was lower than that of the negative group (P < 0.05), while that of the Caspase-3 positive patients was higher than that of the negative group (P < 0.05). Conclusion: The expressions of Survivin and Caspase-3 in HCC can reflex the biological behavior of HCC and patients prognosis, they may be new targets of tumor diagnosis and treatment.
Objective： The aim of our study was to investigate the expressions of Survivin and Caspase-3 in the hepato- cellular carcinoma （HCC） and the paracancerous liver tissues, and define the relationship between the expression and the patients survival rate after surgery. Methods： The expressions of PCNA, Survivin and Caspase-3 were detected by immuno- histochemistry. PCNA positive level was used to account proliferating cell nuclear antigen proliferating index （PCNA-PI）. The apoptosis index of HCC tissues was detected by TUNEL. Results： The expression of Survivin in HCC tissue was higher than that in paracancerous tissue （P 〈 0.05）, while it was related with portal vein thrombosis, tumor size and histological differentia- tion （P 〈 0.05）. In HCC tissues, the PCNA-PI of the Survivin positive group was higher than the negative （P 〈 0.05） group, and the AI of the Survivin positive group was lower than the negative group （P 〈 0.05）. The expression of Caspasse-3 in HCC tissue was lower than that in paracancerous tissue （P 〈 0.05）, while it was related with HBsAg and histological differentiation （P 〈 0.05）. In HCC tissues, the average PCNA-PI of the Caspase-3 positive group was lower than that of the negative group （P 〈 0.05）, and the average AI of the Caspase-3 positive group was higher than that of the negative group （P 〈 0.05）. There was negative correlation between the expressions of Survivin and Caspase-3 in HCC （r = -0.242, P 〈 0.05）. The survival rate of the Survivin positive group was lower than that of the negative group （P 〈 0.05）, while that of the Caspase-3 positive patients was higher than that of the negative group （P 〈 0.05）. Conclusion： The expressions of Survivin and Caspase-3 in HCC can reflex the biological behavior of HCC and patients prognosis, they may be new targets of tumor diagnosis and treatment.
