榼藤子生品与炮制品HPLC指纹图谱研究

Study on HPLC fingerprint of crude and processed products of Entada phaseoloides

目的:建立榼藤子生、炮制品HPLC指纹图谱的评价方法,分析各样品指纹图谱的变化。方法:采用HPLC-UV分别对榼藤子生品和炮制品的指纹图谱进行研究,用HPLC-ESI-MS对其生品共有峰进行鉴定。结果:标示出生品16个共有峰,炮制品21个共有峰,应用HPLC-MS和对照品保留时间的对应初步鉴定了其中9个共有峰,主要为榼藤子三萜皂苷类和其他糖苷类成分的吸收峰。结论:榼藤子生品炮制后,60%甲醇提取液中部分化学成分含量有不同程度的下降或上升,指纹图谱的主峰数目也有变化,说明该法可以较全面地反映榼藤子炮制前后化学成分的差异,用于控制生品和炮制品的内在质量。

Objective： A novel method was established and the results were compared for HPLC fingerprint determination of crude and processed products of Entada phaseoloides.Method： HPLC-UV was proposed for studying the fingerprints of fresh E.phaseoloides and their processed products,respectively.HPLC-ESI-MS was introduced to analyze the common peaks in each batch of crude E.phaseoloides.Result： Sixteen characteristic peaks were found in crude E.phaseoloides samples and twenty-one common peaks existed in processed E.phaseoloides samples.Nine characteristic peaks of which were identified by comparison of the retention time and their molecular weights of chemical standards,most of which were identificated belong to triterpenoid saponins and glucosides.Conclusion： After processed,the chemical composition of the extraction with solution of 60% methanol from crude E.phaseoloides are less or more than that from processed E.phaseoloides,and the changes of the main peaks of fingerprint chromatographic suggest that HPLC can be used to reflect the difference of chemical composition of E.phaseoloides and their processed products.It would be an efficient way for qualitative control of E.phaseoloides.