马兰化学成分及生物活性研究

Chemical constituents and bioactivity of Kalimeris indica

目的:研究马兰的化学成分及生物活性。方法:利用多种柱色谱分离技术分离纯化化合物,用各种波谱数据解析鉴定化合物的结构;采用平板打孔法,通过观察样品是否产生抑菌圈及抑菌圈大小,半定量地对样品的抗菌活性做出评价。结果:从马兰的乙醇提取部分分离得到8个化合物,分别鉴定为琥珀酸(1),1-O-十六烷酸甘油酯(2),原儿茶酸(3),尿嘧啶(4),丁香酸(5),原儿茶酸甲酯(6),七叶内酯(7),苜蓿酸(8);活性筛选结果表明:琥珀酸(1)和丁香酸(5)对枯草芽孢杆菌有抑制作用。结论:所有化合物均为首次从该属植物中分离得到,琥珀酸和丁香酸对枯草芽孢杆菌有抑制作用。

Objective：To study the constituents of Kalimeris indica and its bioactivity.Method：The constituents were isolated by various chromatographic techniques and their structures were elucidated by their physicochemical properties and the spectral data analysis.Use the disk diffusion method,see whether a sample can produce inhibition zone and its round size,semi-quantitatively evaluate the samples antimicrobial activity.Result：Eight compounds were isolated and identified as succinic acid（1）,glycerolmonopalmitate（2）,protocatechuic acid（3）,uracil（4）,syringic acid（5）,protocatechuic acid methyl ester（6）,esculetin（7）,medicagenic acid（8）.The result of activity screening indicate that succinic and syringic acid have bacteriostasis to bacillus subtilis.Conclusion：All Compounds were isolated from the genus Kalimeris for the first time,succinic acid（1） and syringic acid（5） have bacteriostasis to bacillus subtilis..