香蕉NPR1基因的克隆及植物表达载体构建

Clonging and Construction of a Plant Expressing Vector of NPR1 Gene from Banana

病程相关基因非表达子（NPR1）是植物抗病信号传导的关键基因。以香蕉抗病香蕉品种为材料．根据NPR1基因序列在保守区设计引物．从香蕉cDNA中克隆获得NPR1基因片段．以RACE的方法克隆获得香蕉NPR1基因的全长，并命名为GCT119-NPR1。香蕉GCT119-NPR1基因最大阅读框（ORF）为1707bp。生物信息学分析结果表明，该基因含有ANK和BTB两个结构域．其中ANK结构域是NPR1基因中非常重要的结构域，在蛋白质相互作用中起着至关重要的作州。同源性分析表明．该基因与已克隆发表的NPR1基因氨基酸序列同源性为67％～82％。将其构建植物表达载体．为下一步转化工作奠定基础。

The NPR1 （nonexPressor of pathogenesis-related genes 1） gene is a key gene involed in regulation of plant disease resistance. A pair of primes were designed based on the conserved domain of the NPR1 gene. A full-length eDNA of NPRI gene was isolated from resistance banana of Fusariurn oxysporum by application of PCR and rapid amplification of eDNA （RACE） techniques. The amplified product of GCT119-NPR1 was cloned and sequenced. The deduced amino acids of this sequence shared a high homology. Blast showed that the amino acids sequence presented a high homology （67%-82%） with the NPR1 gene in other plants. And the plant express vector containing NPR1 gene was constructed.