流动注射-光度法测定超氧阴离子自由基(O_2^-·)与超氧化物歧化酶(SOD)活性的研究

Studies on the Determination of Superoxide Anion Radical(O-2) and Superoxide Dismudase(SOD) Activity by Flowinjection Spectrophotometry

合成了４种硫代碳酰腙类化合物双水杨醛硫代碳酰腙（ＢＳＴＺ）、双对羟基苯甲醛硫代碳酰腙（ＢＨＢＴＺ）、双香兰素硫代碳酰腙（ＢＭＨＢＴＺ）和双邻香兰素硫代碳酰腙（ＢＭＳＴＺ），其中苯环醛基对位具有羟基的ＢＨＢＴＺ和ＢＭＨＢＴＺ能与超氧阴离子自由基（Ｏ－·２）、Ｈ２Ｏ２发生显色反应，而且ＢＭＨＢＴＺ与Ｏ－·２的反应灵敏度为最高，由此建立了流动注射－光度法测定超氧阴离子自由基（Ｏ－·２）的方法．此方法的线性范围为４．５×１０－７～１．５×１０－６ｍｏｌ／Ｌ，线性方程为Ａ６９８＝１．８×１０４ｃ（Ｏ－·２）＋０．００４５，相关系数ｒ＝０．９９８４，检出限为１．０×１０－７ｍｏｌ／Ｌ．相对标准偏差为５．２３％．

Four thiocarbohydrazones of 1,5bis(salicylidene)thiocarbohydrazone(BSTZ), 1,5bis(phydroxybenzaldene)thiocarbohydrazone(BHBTZ), 1,5bis(mmethoxyphydroxybenzaldene)thiocarbohydrazone(BMHBTZ) and 1,5bis(mmethoxysalicylidene)thiocarbohydrazone(BMSTZ) were synthesized. BHBTZ and BMHBTZ can be oxidated by superoxide anion radical(O-2) and H2O2. Based on the reaction, the determination of O-2 by flow injection spectrophotometry was developed. The linear range of O-2 concentration is from 4510-7 mol/L to \{15\}10-6 mol/L. The detection limit is 1510-7 mol/L. The relative standard deviation is 523%. Coexist substances in many biological samples have no interference. The method can also be used to determine the activity of superoxide dismudase(SOD).