野生和人工栽培漆树液多糖的分子结构与生物活性

Structure and Bioactivities of Lacguer Polysaccharides from Chinese Lac Trees of Wild Species and Cultispecies

从湖北毛坝野生（大木）和人工栽培（小木）漆树液中分离纯化出酸性杂多糖Ｄ－ＬＰ和Ｘ－ＬＰ，化学降解和光谱分析表明两种多糖均含有Ｄ－Ｇａｌ、Ｄ－ＧｌｃＡ、Ｌ－Ａｒａ和Ｌ－Ｒｈａ，其摩尔比分别为７７∶１８．３∶３．７∶１和６３．７∶１３．３∶３．９∶１．两种漆多糖都是以β（１→３Ｄ－Ｇａｌ为主链的高度支化酸性杂多糖．Ｄ－ＬＰ支化度较高，分支点在Ｇａｌ的Ｃ６和Ｃ４位；而Ｘ－ＬＰ仅有部分主链Ｄ－Ｇａｌ的Ｃ６位发生支化．小鼠腹腔注射试验表明，两种漆多糖均具有明显的免疫抑制作用。

Two lacguer polysaccharides(LP) were isolated and purified from sap of Damu(wild species) and Xiaomu(cultispecies) lac trees in Hubei Province of China, and were analyzed by \{using\} chemical degradation, FTIR, GC, GCMS, 1H NMR and HPGPC. The results indicated that two LP were all composed of (13DGal main chain with the branches having \{(16)\} linked glucuronic acid, arabinose and rhamnose. Differently, the LP from Damu contain branches linked with (14) and higher branching factor than that of Xiaomu. The molecular weight (M w) was obtained to be 10.7104 and 12.5104 by HPGPC, respectively. The results \{obtained\} by intra perctoncal injection in mice showed that DLP and XLP have immunostimulant activities, and the DLP from Damu has a significantly higher bioactivity in motivating the growth of leucocytes than that of Xiaomu. It is regarded that the decrease in the molecular weight could lead to an enhancement of bioactivities of the DLP from Chinese lac tree of wild species.\=