连续逆向色谱电泳过程

CONTINUOUS COUNTERACTING CHROMATOGRAPHIC ELECTROPHORESIS PROCESS

连续CACE过程借助流场和逆向电场的共同作用,使目标蛋白质在分离柱富集区内连续富集。本文提出了连续CACE分离柱内浓度分布微分方程和定解条件,并对方程进行了求解。解决了富集区内目标蛋白质浓度的在线检测,用牛血红蛋白和细胞色素C在两套连续CACE装置上进行实验研究,取得了预期效果。

Continuous counteracting chromatographic electrophoresis(CACE) concentrates target-protein continuously to accumulation zone between exclusive and inclusive zones with a separation column by using opposing electrical and flow fields. The mathematical models for three zones of a continuous CAGE column are proposed. An on - line monitor system is constructed to monitor in situ the concentration of target protein and its variation within the accumulating zone. Experiments are made with hemoglobin and cytochrome C by using two sets of continuous CACE apparatus.