小刺豆荚软珊瑚甾醇类化合物的提取、分离及鉴定

Study on the Steroid Constituents of Soft Coral Lobophytum Microspiculatum

从西沙群岛海区采集的小刺豆荚软珊瑚（Lobophytummicrospiculatum）中，通过乙醇提取、乙酸乙酯-水分配、硅胶色谱分离和GC／MS分析等方法，分离、鉴定出11种甾醇。其中柳珊瑚甾醇（11）的质量分数占65.6％。22，24-二甲基-胆甾-5，22（23）-二烯-3β-醇（4）是首次从自然界发现的新化合物，它的发现为柳珊瑚甾醇的生源假说提供了进一步佐证。

The soft coral Lobophytum microspiculatum collected from Xisa Islands of the South China Sea was immersed in and extracted with ethyl alcohol and the extract was concentrated,and then partitioned between ethyl acetate and water. The portion soluble in ethyl acetate was subjected to column chromatography over Silica H, eluted with a step gradient of EtOAc and petroleum ether. The sterols were obtained from 20% EtOAc-petroleum ether eluent portion. By means of GC/MS method, the sterols were identified to be:cholesta-26-methyl-ol; ergosta-8, 22-dien-3β-ol, 24-methyl; ergosta-5, 22-dien-3β-ol，23, 24-dimethyl;cholesta-5, 22(23)-dien-3β-ol, 22, 24-dimethyl; cholesterol; ergosta-5,22-dien-3β-ol, 24-methyl; ergosta-5,22-dien-3β-ol, 22, 24-dimethyl, 24-methyl-cholesterol；ergosta-7-en-3β-ol, 24-methyl；nor-ergosta-7-en-3βol and gorgosterol. Among them cholest-5, 22(23)-3β-ol, 22, 24-dimethyl (4) is a new compound which was found at the first time from the nature. This finding gave a further prove for the biogenesis path way of gorgosterol。