吸附固定相开管毛细管电色谱方法的建立（英文）

METHOD DEVELOPMENT OF ADSORBED STATIONARY PHASE OPEN TUBULAR CAPILLARY ELECTROCHROMATOGRAPHY

首次将管壁吸附作用作为开管毛细管电色谱固定相制备的推动力，成功地建立了称为“吸附固定相开管毛细管电色谱”的一种新方法。原理是：选择合适的条件，让荷正电的化合物在毛细管管壁上充分吸附，直接用吸附层作为固定相。目前，已有数类化合物被用作固定相物质，其中包括阳离子表面活性剂如十六烷基三甲基溴化铵（CTAB）、碱性蛋白质如溶菌酶和细胞色素C、碱性小肽如赖氨酸-酪氨酸和赖氨酸-丝氨酸-酪氨酸、以及碱性氨基酸如L-赖氨酸。CTAB吸附固定相用于分离电中性化合物，其它吸附固定相用于手性分离。所建立的方法重现性好（迁移时间次间RSD值小于2．3％），柱效高（最高柱效可达590000／m，平均柱效在250000／m以上），寿命较长（2～8℃下保存，至少30d）。与已有的固定相制备方法比较，所建立的方法具有简便、快速和稳定等优点。

The adsorption effect of capillary wall, which was usually considered as a troublesome factor, has been used as driving force for preparing stationary phase for open tubular capillary electrochromatography (OTCEC). A number of compounds have been applied as stationary phase materials, which include cationic surfactants such as cetyltrimethylammoniurm bromide (CTAB ), bas ic proteins such as lysozyme and cytochrome C,basic peptides such as Lys-Tyr and Lys-Ser-Tyr, and basic amino acid L-lysine. The adsorbed CTAB phase is used for separation of neutral compounds while other adsorbed stationary phases are used for chiral separation.The run-to-run reproducibility of retention time is rather good with RSD values less than 2. 3 %. The separation efficiency is excellent with the highest theoretical plate number of up to 590 000/m and the average plate number of more than 250 000/m. I3eing stored at 2-8 C in refrigerator, the adsorbed stationary phase can last at least one month.