反相离子对高效液相色谱法快速分离和定量测定食品中的甜蜜素

A Rapid Separation and Quantitation of Sodium Cyclamate in Food by Ion-Pair Reversed-Phase High Performance Liquid Chromatography

采用反相离子对高效液相色谱法快速分离和测定食品中的甜蜜素。在ODS柱上，以V（甲醇）：V（水，含离子对试剂）=30：70的溶液为流动相进行分离，分别考察了流动相中离子对试剂和甲醇浓度对甜蜜素保留行为的影响。检测波长为205nm；采用外标法定量，测得甜蜜素在0．5～2．5g／L范围内具有良好的线性关系；回收率在96．9％～101．7％之间；检测限为0．05g／L。

A method of separation and quantitation of sodium cyclamate in foods by ion-pair reversed-phase high performance liquid chromatography is described. Sodium cyclamate can be separated on ODS column at room temperature by using a mobile phase of V(methanol): V(water, containing ion-pair reagent) =30:70at 1 mL/min now rate. The effects of ion-pair reagent and methanol on the retention of sodium cyclamate were studied. The samples were quantified with an ultraviolet detector operated at 205 urn. The calibration curve was linear in the range of 0. 50-2. 50 g/L with a correlation coefficient of 0. 999 6. The recoveries were 96. 9%～101.7%. The detection limit was 0. 05 g/L. This method is simple, rapid and sensitive.