高效液相色谱法测定蔬菜中利佛米及其主要代谢物的残留量

Determination of Triflumizole and its Metabolite Residues in Vegetables by High Performance Liquid Chromatography

用甲醇提取试样中的利佛米及其代谢产物[4－氯，-a，a,a-三氯代-N－（1-氨-2-丙氧基亚乙基）－O-甲苯胺]，用二氯甲烷反提取，蒸干二氯甲烷层，其残留物用流动相溶解，供配有紫外检测器的高效液相色谱仪测定，外标法定量。分别测定利佛米和其主要代谢物后再计算以利佛米计的总残留量。

Triflumizole (TRI) and its metabolite [4-chloro-a,a,a-trifluoro-N-(1-amino-2-propoxythylidene ) O-toluidine] (MET) were extracted with methanol and reverse-extracted with dichloromethane. After evaporation to dryness, the residue in dichloromethane layer was dissolved in mobile phase for high Performance liquid chromatographic determination with external standard method. The total residue content of TRI and MET (calculated as TRI) is reported.