摘要
建立了蜂体中10－HDA的高效液相色谱分析方法。对10－HDA的提取方法进行了研究。在选择的最佳色谱条件下，线性范围为10～1000ng，r=0．9998，回收率96．5％～99．2％，检测限为0.53μg／g。
In the present work we found that in the honeybee body there exists an unsaturated fatty acid,trans-10-hydroxy-2-decencic acid (10-HDA), which was known only to be present in royal jelly. We established the analytical method of 10-HDA in honeybee body by HPLC and simplified the extraction method of 10-HDA. In the optimum conditions the linear range of detection was 10-1000 ng, the correlation coefficient was 0. 999 8, the recovery was 96. 5%-99.2% and the detectable limit was 0. 53 μg/g.
出处
《色谱》
CAS
CSCD
北大核心
1999年第3期301-302,共2页
Chinese Journal of Chromatography
关键词
高效液相色谱法
蜜蜂蜂体
王浆酸
high performance liquid chromatography
honeybee body
10-HDA
