HPLC/CoPC修饰电极安培检测大鼠心肌线粒体中谷胱甘肽的研究

The Study on Determination of the Glutathione in Myocardial Mitochondrion with HPLC/CoPC Modified Electrode

研究了钴酞菁（ＣｏＰＣ）修饰电极的修饰方法及其电化学性质。它可以催化氧化谷胱甘肽（ＧＳＨ），降低ＧＳＨ的过电位。以ＣｏＰＣ化学修饰电极为工作电极的安培薄层电化学检测器，与高压液相色谱（ＨＰＬＣ）联用，在工作电极电位为＋０．８Ｖ时，ＧＳＨ的浓度在３．０×１０－６ｍｏｌ／Ｌ～１．０×１０－３ｍｏｌ／Ｌ浓度范围内与峰电流呈良好的线性关系，线性相关系数为０．９９９１，检出限为１．０×１０－６ｍｏｌ／Ｌ。将该方法应用于安静、力竭及恢复三种运动模型的大鼠心肌线粒体中谷胱甘肽的测定，获得满意的结果。该方法简便、快速，且灵敏度较高，为电分析化学在生物学及运动生理学等生命科学学科的应用提供了新的手段。

In this paper, a new cobalt phthalocyanine(CoPC) electrode and its electrochemical properties are studied. It can electrocatalyze glutathione(GSH), increase the sensitivity and lower overpotential. The CoPC modified electrode is used as the working electrode of amperometric detector of HPLC, and the working potential is +0.8V(vs. Ag/AgCl). The linear correlation coefficient is 0.9991 in range of 3.010-6 mol/L to 1.010-3 mol/L with a detection limit of 1.010-6 mol/L. The application of this method in the determination of GSH in myocardial mitochondrion of rats gives an excellent result.