胶束电动毛细管电泳同时测定饮料中的多种添加剂

Simultaneously Determination of Five Additives in Beverage by Micellar Electrokinetic Capillary Chromatography

建立了分析饮料中山梨酸、苯甲酸、１０－羟基癸烯酸、咖啡因和糖精钠五种添加剂的胶束电动毛细管电泳法（ＭＥＣＣ）。讨论了电压、缓冲溶液浓度和ｐＨ值、胶束浓度对分析结果的影响。５种添加剂的迁移时间和测定回收率的变异系数分别小于１．５％和５％，检测限分别为１０，１０，５，１０，２０μｇ／ｋｇ。

A useful method for determination of benzoic acid, sorbic acid, saccharin, caffeine, and 10hydroxydencenoic acid in beverage by micellar capillary chromatography has been developed. Experiments were performed with 30 kV power, 50m57cm fusedsilica capillary column and the absorption detection was at 225nm. Sample were injected into the capillary by applying pressure for 5s and the running buffer was made up of 50mmol/L sodium dodecyl sulfate (SDS) and 25mmol/L borate (pH 9.2). The influence of operation voltage, pH of buffer, SDS concentration on the additives migration has been studied. The coefficients of variation of migration time and the recoveries of 5 additives were below 1.5% and 5%, respectively. The detection limits for five additives are 10, 10, 5, 10, 20g/kg, respectively.