理化因子对嗜盐隐杆藻细胞生长及胞外多糖产量的影响

EFFECTS OF GROWTH CONDITIONS ON GROWTH RATE AND\= EXOPOLYSACCHARIDE PRODUCTIONS BY APHANOTHECE HALOPHYTICS

比较不同ＮａＣｌ、Ｃａ２＋、ＰＯ３－４等离子浓度对嗜盐隐杆藻（Ａｐｈａｎｏｔｈｅｃｅｈａｌｏｐｈｙｔｉｃａ）细胞生长及胞外多糖（Ｅｘｏｐｏｌｙｓａｃ－ｃｈａｒｉｄｅＥＰＳ）产量的影响。在各影响因子不同浓度的培养条件下，０．５ｍｏｌ／Ｌ的ＮａＣｌ、１．０ｇ／Ｌ的Ｃａ（ＮＯ３）２·４Ｈ２Ｏ、０．１ｇ／Ｌ的ＫＨ２ＰＯ４分别是其最佳生长浓度。ＥＰＳ的产量在０．５ｍｏｌ／ＬＮａＣｌ、，０．５ｇ／ＬＣａ（ＮＯ３）２·４Ｈ２Ｏ、０．５ｇ／Ｌ的ＫＨ２ＰＯ４培养条件下最高。在较低的Ｃａ２＋、Ｍｇ２＋、ＰＯ３－４浓度下可提高ＥＰＳ产率。

The cell growth and EPS production are investigated under different growth conditions by changing some nutritional and physicalchemical parameters known to affect the synthesis of expolysaccharides and cell number. \{Among\} the factors tested, 0.5 mol/L NaCl, 1.0 g/L Ca(NO\-3)\-24H\-2O, 0.10 g/L KH\-2PO\-4 are suitable for cell growth concentration and alga can produce more EPS in culture of 0.5 mol/L NaCl, 0.5 g/L Ca(NO\-3)\-24H\-2O, \{0.05\} g/L KH\-2PO\-4. Limitation of Ca\+\{2+\}, PO\+\+\{3-\}\-\-4 all can induce a significant enhancement of EPS synthesis.