肝硬化对大鼠异丙酚镇静效力的影响

Effect of liver cirrhosis on potency of propofol for sedation in rats

目的 探讨肝硬化对大鼠异丙酚镇静效力的影响.方法 健康雄性SD大鼠58只,体重180～220 g,随机分为3组,正常对照组（C组,n=18）、轻度肝硬化组（M1组,n=20）和重度肝硬化组（M2组,n=20）.M1组和M2组采用四因素法制备大鼠轻度肝硬化和重度肝硬化模型.模型制备成功后,静脉注射异丙酚,第1只大鼠的剂量为5.912 mg/kg,应用序贯法确定下一只大鼠的剂量,相邻剂量比为0.85,以翻正反射消失作为判断异丙酚镇静起效的标准.采用序贯法计算异丙酚镇静效应的半数有效剂量（ED50）.结果 与C组比较,M1组及M2组异丙酚镇静效应的ED50降低（P＜0.05或0.01）;与M1组比较,M2组异丙酚镇静效应的ED50降低（P＜0.05）.结论 肝硬化可强化大鼠异丙酚的镇静效力.

Objective To investigate the effect of liver cirrhosis on the potency of propofol for sedation in rats. Methods Fifty-eight male SD rats, aged 10-12 weeks, weighing 180-220 g, were randomly divided into 3 groups： control group （group C, n = 18）, mild liver cirrhosis group （group M1, n =20） and severe liver cirrhosis group （group M2, n = 20）. The model of liver cirrhosis was established using four factors described by Chen et al. After successful establishment of the model, propofol was injected intravenously. The dose of propofol was determined by up-and-down sequential method for loss of righting reflex. The dose of propofol was 5.912 mg/kg in the first rat and the ratio of the doses between the two consecutive rats was 0.85. ED50 of propofol was calculated using up-and-down sequential method. Results ED50 of propofol was significantly lower in group M1 and M2 than in group C and in group M2 than in group M1 （ P ＜ 0.05 or 0.01 ）. Conclusion The liver cirrhosis can enhance the potency of propofol for sedation in rats.