摘要
本文报道了Tb-吡哌酸生成络合物后,在紫外光照射下发生分子内能量传递使Tb产生特征荧光,其荧光强度与吡哌酸的浓度成线性关系,由此建立了一种灵敏、快速的分析吡哌酸的方法。此方法具有背景干扰小,选择性高和体系中铽的荧光寿命长等特点。尤其适用于血清、尿样等体液分析,回收率在94.6%~102%。该方法检出限为3.6ng/mL。其线性范围是5.0×10-8~4.0×10-6mol/L。
Intramolecular energy transfer occurs in the terbium complex with pipemidic acid(PPA) and the terbium(Ⅲ) ion fluorescence can be sensitized by PPA.The fluorescence intensity of the Tb 3+ PPA system is proportional to the amount of PPA.Accordingly,a fluorimetric method for the determination of PPA is proposed.The characteristics of the method include low background noise,high selectivity and long fluorescence lifetime.The method can be applied to the determination of PPA in urine and serum.The recovery is in the range of 94 6% 102%.Its detection limit and linear range for PPA are 3 6 ng/mL and 5 0×10 -8 4 0×10 -6 mol/L,respectively.
出处
《光谱学与光谱分析》
SCIE
EI
CAS
CSCD
北大核心
1999年第3期447-449,共3页
Spectroscopy and Spectral Analysis
基金
国家自然科学基金
攀登计划资助项目
关键词
荧光光度法
铽
测定
吡哌酸
血清
尿
Terbium, Pipemidic acid, Fluorimetry, Serum, Urine