Tb-吡哌酸的荧光体系及尿和血清中吡哌酸含量的测定

Study of the Fluorescence of Terbium Pipemidic Acid System and the Determination of Pipemidic Acid in Urine and Serum

本文报道了Ｔｂ－吡哌酸生成络合物后，在紫外光照射下发生分子内能量传递使Ｔｂ产生特征荧光，其荧光强度与吡哌酸的浓度成线性关系，由此建立了一种灵敏、快速的分析吡哌酸的方法。此方法具有背景干扰小，选择性高和体系中铽的荧光寿命长等特点。尤其适用于血清、尿样等体液分析，回收率在９４．６％～１０２％。该方法检出限为３．６ｎｇ／ｍＬ。其线性范围是５．０×１０－８～４．０×１０－６ｍｏｌ／Ｌ。

Intramolecular energy transfer occurs in the terbium complex with pipemidic acid(PPA) and the terbium(Ⅲ) ion fluorescence can be sensitized by PPA.The fluorescence intensity of the Tb 3+ PPA system is proportional to the amount of PPA.Accordingly,a fluorimetric method for the determination of PPA is proposed.The characteristics of the method include low background noise,high selectivity and long fluorescence lifetime.The method can be applied to the determination of PPA in urine and serum.The recovery is in the range of 94 6% 102%.Its detection limit and linear range for PPA are 3 6 ng/mL and 5 0×10 -8 4 0×10 -6 mol/L,respectively.