双荧光剂荧光光度法研究与应用——四溴荧光素·丁基罗丹明B体系

Study on the Double Fluorescers Fluorometry and Its Application

向离子缔合物ＧａＣｌ４ＢＲＢ（ＢＲＢ是丁基罗丹明Ｂ）的甲苯萃取液中，加入ｐＨ＝８．７的ＮａＯＨ－ＫＨ２ＰＯ４缓冲溶液，离子缔合物解析后ＢＲＢ进入水相。向其中加入与ＢＲＢ颜色相近的四溴荧光素（ＴＢＦ），形成的ＢＲＢ２ＴＢＦ再被甲苯萃取，萃取液中加入正丁醇（甲苯：正丁醇＝１１）。因萃取液中ＢＲＢ２ＴＢＦ［１］的双荧光剂协同作用，提高了测定镓的灵敏度。

Buthylrhodamine B(BRB) was produced and entered into aqueous phase when pH=8 7 buffer solution was added to toluene extractive solution of GaCl 4BRB +.And then BRB 2·TBF was extractred by tetrabromofluorescein(TBF).N butanol(toluene:n butanol=1∶1)was added to toluene extractive solution of BRB 2.TBF.The sensitivity was raised because of the assistant effect of BRB 2·TBF.The detection limit was 0 1 μg/L.Beer′ law was obeyed in the range of 0 0 15 mg/L with a relative standard deviation of 3 9% for 0 002 7% gallium in metal and the recoveries were 94 7% 105 2%.