不同采收时期内野老鹳草中活性成分的动态变化研究

Study on the Dynamic Variation of Active Components in Geranium carolinianum from Different Collection Time

目的:测定不同采收时期野老鹳草中活性成分没食子酸及鞣花酸的含量,为最佳采收期的确定提供依据。方法:采用HPLC对不同采收期的野老鹳草中没食子酸及鞣花酸的含量进行测定。色谱条件:色谱柱为Dia-monsil C18色谱柱(150 mm×4.6 mm,5μm);流动相为乙腈-0.1%磷酸溶液梯度洗脱;流速为1.0 mL/min;检测波长为274 nm;柱温为25℃。结果:没食子酸及鞣花酸的线性范围分别是:0.075～5.00μg(r=0.9995)、0.05～2.00μg(r=0.9995),平均回收率分别是99.88%(RSD=1.19%)、99.08%(RSD=2.81%)。结论:野老鹳草中没食子酸及鞣花酸的含量均在开花期开始上升,于始果期达到最高。

Objective： To determine the contents of the active components,gallic acid and ellagic acid in Geranium carolinianum from different collection time and to define the best collection time for this herb.Methods： The contents of gallic acid and ellagic acid in each samples of Geranium carolinianum were determined by HPLC.The HPLC method was performed on a Diamonsil C18 column（150 mm × 4.6 mm,5 μm） with acetonitrile-0.1% H3PO4 as mobile phase.The flow rate was 1.0 mL/min,the detection wavelength was 274 nm and the column temperature was 25 ℃.Results： The calibration curve of gallic acid and ellagic acid were linear in the range of 0.075 ～ 5.00 μg（r = 0.9995） and 0.05 ～ 2.00 μg（r = 0.9995）,respectively.The average recovery of gallic acid and ellagic acid were 99.88%（RSD = 1.19%） and 99.08%（RSD = 2.81%）,respectively.Conclusion： The content of gallic acid and ellagic acid in Geranium carolinianum both began to increase in flowering stage and increased to the maximum value in immature-fruit stage.