云威灵炮制过程中化学成分变化研究

Studies on the Changes of Chemical Constituent in Inula nervosa During the Processing

目的:研究云威灵在炮制过程中化学成分的变化。方法:采用高效液相色谱法检测云威灵不同条件炮制品,以乙腈-水为流动相,梯度洗脱,检测波长280 nm。以50%乙醇提取,用色谱方法分离纯化新产生的化合物1,根据理化性质和光谱分析鉴定化学结构。结果:云威灵炮制前后高效液相色谱图发生变化。从炮制品中分离到1个新产生的化合物单体,鉴定为5-羟甲基糠醛。结论:云威灵在炮制前后化学成分发生变化,首次从该属植物中分离到5-羟甲基糠醛。

Objective： To research the changes of chemical constituent in Inula nervosa during the processing.Method： Inula nervosa processed with different conditions was determined by HPLC,with acetonitrile-water as a mobile phase using gradient elution,and the detective wavelength was 280 nm.The emerging compounds 1 was extracted by 50% ethanol,isolated and purified using chromatography,and identified according to the physicochemical properties and spectral analysis.Results： The HPLC chromatogram of Inula nervosa changes before and after processing.A monomer compounds was isolated from processed Inula nervosa.It was Identified as 5-hydroxymethylfurfural.Conclusion： The chemical constituent of Inula nervosa changes during the processing.It is the first time that compound 1 were isolated from Inula。