银杏酮酯磷脂复合物的制备与理化性质的研究

Preparation of Ginkgo biloba Extract 50-Phospholipid Complex and Study on Its Physicochemical Properties

目的:制备银杏酮酯的磷脂复合物并优化制备工艺,对其进行理化性质的考察。方法:以黄酮醇苷与大豆磷脂的复合率为指标,进行单因素考察,并用正交试验优化制备工艺。对银杏酮酯磷脂复合物进行DSC和IR理化性质的鉴定,测定其在不同pH的溶液中的表观油水分配系数。结果:以四氢呋喃为反应溶剂,反应温度为30℃,反应物质的浓度为20 mg/mL,反应物质的重量比为1∶1时反应生成的复合物复合率达98%,并且银杏酮酯磷脂复合物明显改善了银杏酮酯在正辛醇中的溶解性能,在不同pH水-正辛醇系统中,银杏酮酯磷脂复合物的表观油水分配系数与银杏酮酯相比也有差异。结论:银杏酮酯磷脂复合物的理化性质相比银杏酮酯有较显著的改变。

Objective： To optimize the preparation technology of GBE 50-phospholipid complex and study its physicochemical properties.Methods： The preparation conditions for GBE 50-phospholipid complex were optimized by means of single factor study and orthogonal design,and taking the complexing rate of total flavonoids as assessmant criteria,the complex was analyzed by DSC,IR and determined apparent oil-water distribution coefficients in different pH aqueous solution.Results： The optimized preparation conditions for GBE 50-phospholipid complex were obtained as follows： the solvent was Tetrahydrofuran,the temperature was 30℃,the concentration of GBE 50 was 20 mg/mL,the ratio of GBE 50 to phospholipids was 1 to 1,and the complexing rate was 98%.The complex significantly improved GBE 50 on the solubility in octanol,also on the oil-water apparent partition coefficient.Conclusion： GBE 50-phospholipid complex is very different from GBE 50 on the physicochemical properties.