响应面法优化伏毛铁棒锤根部总生物碱提取工艺

Optimization of Extraction Process of Total Alkaloids from Radix of Aconitum flavum Using Response Surface Methodology

目的:研究伏毛铁棒锤根中总生物碱的最佳提取工艺。方法:采用3因素3水平响应面法对伏毛铁棒锤总生物碱乙醇回流提取工艺进行了优化。结果:建立了一个总生物碱得率与影响因子的多元二次回归方程,得到总生物碱的最佳提取工艺条件:提取时间1.72 h,乙醇浓度64.00%,料液比8.18 mL/g,总生物碱得率的预测值可达0.526%,实测值为0.521%,与预测值非常接近。结论:二次多项式建立的数学模型具有良好的预测性,所选工艺条件重现性好。

Objective： To study the technological parameters of the extraction process of total alkaloids from Radix of Aconitum flavum.Methods： Using response surface methodology（RSM） of three factors and three levels to optimize ethanol heat reflux applied for alkaloids extraction from Radix of Aconitum flavum.Results： A quadratic polynomial mathematical model with good correlation was constructed and employed to the optimization.The optimal extraction conditions were as follows： the extraction time was 1.72h,the concentration of ethanol was 64.00%,the ratio of ethanol to raw material was 8.18 mL/g,under which the predictive value of the rate of extraction yield of alkaloids was 0.526% and the measured value was 0.521%,respectively,and there was a good agreement with their predicted values.Conclusion： This reveals the good predictability of the mathematical model,the selected process also has a good reproducibility.