人工蛹虫草子实体不同提取物的致突变作用

A Study on Mutagenesis of Artificial Cordyceps Militaris Fruitbody Extracts

[目的]研究人工蛹虫草子实体水提物和醇提物的致突变可能性。[方法]以经口灌胃方式,分别给予小鼠不同剂量的人工蛹虫草子实体水提物和醇提物,观察其对小鼠骨髓细胞微核率和小鼠精子畸形率的影响;以不同剂量的人工蛹虫草子实体水提物和醇提物稀释液对体外哺乳动物细胞(CHL)进行处理,观察其对CHL染色体畸变率的影响。[结果]2.5、5.0、10.0g/kg组小鼠的骨髓细胞微核率和精子畸形率与阴性对照组比较,差异均无统计学意义(P>0.05);在加和不加代谢活化系统条件下,156.0、321.0、625.0、1250.0、2500.0μg/mL浓度组的CHL染色体畸变率与阴性对照组比较,差异均无统计学意义(P>0.05)。[结论]在本研究条件下未发现两种人工蛹虫草子实体提取物具有直接或间接的致突变作用。

[Objective] To study possible mutagenic and genetic toxicity of the Artificial Cordyceps Militaris Fruitbody Water Extract（ACMFWE）and the Artificial Cordyceps Militaris Fruitbody Ethanol Extract（ACMFEE）on mice.[Methods] Total of 120 mice were orally administered with ACMFWE and ACMFEE in various concentrations,micronucleus in bone marrow cells and sperm abnormalities of mice were observed;different concentrations of ACMFWE and ACMFEE were applied to in vitro mammalian cells（CHL）,and the chromosome aberration was observed.[Results] The bone marrow cells micronucleus rates and sperm deformity rates of mice showed no difference compared to the corresponding control groups when treated with 2.5-10.0 g/kg ACMFWE or ACMFEE.With or without the metabolic activation system,the chromosome aberration rates of CHL were also found no significant difference compared to the corresponding control groups when treated with 156.0-2 500.0 μg/mL ACMFWE or ACMFEE.[Conclusion] No direct or indirect mutagenesis or genetic toxicity was found in mice treated with two kinds of artificial Cordyceps fruiting body extract in current study.