牦牛睾丸附睾LDH同工酶表达模式的研究

STUDIES ON LDH ISOZYME EXPRESSION PATTERN IN TESTES AND EPIDIDYMIS TISSUE OF THE YAK

利用比色法和聚丙烯酰胺凝胶电泳（ＰＡＧＥ）技术对６、１２、１８、２４月龄半血野牦牛、横交半血野牦牛和家牦牛睾丸、附睾组织ＬＤＨ同工酶活性及谱带表达进行测析。结果表明，睾丸、附睾ＬＤＨ在相同月龄其表达模式不同，６月龄睾丸ＬＤＨ有四条酶谱，活性百分率依次（ＬＤＨ１＞ＬＤＨ３＞ＬＤＨ２＞ＬＤＨ４），附睾只有三条酶谱（ＬＤＨ１＞ＬＤＨ２＞ＬＤＨ３）；到１２、１８月龄时，睾丸ＬＤＨ有五条酶谱（ＬＤＨ１＞ＬＤＨ３＞ＬＤＨ２＞ＬＤＨ４＞ＬＤＨ５），附睾４条酶谱（ＬＤＨ１＞ＬＤＨ２＞ＬＤＨ３＞ＬＤＨ４），睾丸和附睾ＬＤＨ同工酶随月龄增长和睾丸组织发育，活性和酶谱表现出明显组织器官的特异性。三种类型牦牛ＬＤＨ同工酶表达基本相似。２４月龄时，睾丸、附睾ＬＤＨ有６条谱带，ＬＤＨＸ位于ＬＤＨ４与ＬＤＨ５之间，证明此时牦公牛睾丸中精子成熟。

A polyacrylamide gel electrophoresis (PAGE) technique was used to detect the lactate dehydrogenase (LDH) isozymes in testes and epididymis tissue of half blood wild yak,cross blood wild yak and domestic yak at 6,12,18,24 months of age.The results showed that the LDH of the testes and epididymis were different on its expression pattern at the same age.4 bands (LDH 1>LDH 3>LDH 2>LDH 4) on testes and 3 bands (LDH 1>LDH 2>LDH 3) on epididymis appered at 6 months.5 bands on testes tissue (LDH 1>LDH 3>LDH 2>LDH 4>LDH 5) and 4 bands on epididymis (LDH 1>LDH 2>LDH 3>LDH 4) appered at 12 18 months.Along with age the LDH enzyme of testes and epididymis tissue and germ cell were more and more.The LDH activities and isozyme band were organ specific.There were 6 bands of testes and epididymis LDH appered in the age of 24 months and LDH X located between LDH 4 and LDH 5.All those symbolized the begining of sperm maturity in the testes of yaks.