病毒外壳蛋白基因在马铃薯基因组中的整合与转录表达

The Integration and Transcription of Virus Coat \=Protein Gene in Potato Genome

以表达马铃薯卷叶病毒（ＰＬＲＶ）外壳蛋白（ＣＰ）基因的转基因马铃薯植株为供试材料，在接种病毒后续发感染条件下提取转基因植株ＤＮＡ和ＲＮＡ，用克隆的病毒ＣＰ基因ｃＤＮＡ为探针进行Ｓｏｕｔｈｅｒｎ和Ｎｏｒｔｈｅｒｎ杂交分析，结果表明，外源ＣＰ基因稳定整合于马铃薯基因组内，并在转录水平上高效表达．

The DNA and RNA were extracted from secondary infected transgenic potato plants expressing coat protein(CP) gene of potato leaf-roll virus(PLRV).Southern and Northern hybridization were performed using PLRV coat protein gene cDNA as a probe Results showed that virus coat protein gene has integrated into potato genome.The integrated coat protein gene expressed efficiently at transcription level.\=