摘要
为了从分子水平鉴别三例同核异质小麦雄性不育材料,利用RAPD技术,对细胞核来自同一普通小麦(Triticum aestivum),细胞质分别来自粘果山羊草(Aegilops kotschyi)、二角山羊草(Ae.bicornis)和斯卑尔脱小麦变种(T.spelta)的三例同核异质小麦雄性不育系的线粒体DNA(mtDNA)进行差异片段的比较分析。结果表明:(1)三种不育系在mtDNA上呈现明显差异;(2)对三种不育系的mtDNA差异片段进行回收、克隆、测序和比对,结果片段OPY01-1517和OPP02-750均与普通小麦mtDNA具有较高的同源性,同源性分别为99%和98%。这为进一步从分子水平鉴别含有斯卑尔脱小麦和二角山羊草细胞质的小麦材料奠定了基础,也为区别不同细胞质雄性不育系提供了一定依据。
Comparative analysis of the mitochondrial DNA（mtDNA ） from three isonuclear alloplasmic CMS lines of wheat was conducted by RAPD technique.The results showed：1.Three kinds of wheat CMS lines presented obvious difference in mtDNA.2.Three special fragments abtained from three male sterile lines were successfully cloned and sequenced,the results showed that the sequences of OPY01-1517 and OPP02-750 had higher homology with the mitochondrial DNA of wheat（triticum aestivum）,the homology were 99%and 98%respectively,which provided theoretical and technical support for further identifying the wheat male sterile lines with cytoplasm of T.spelta and Ae.bicornis at the molecular level.
出处
《麦类作物学报》
CAS
CSCD
北大核心
2010年第6期997-1000,共4页
Journal of Triticeae Crops
基金
国家高技术研究发展计划(863计划)重大专项(No.2009AA101102)
高等学校博士学科点专项科研基金项目(20090204110024)
陕西省"13115"科技创新工程重大科技专项(No.2007ZDKG-020)
国家杨凌农业生物技术育种中心专项基金项目(No.99-1A)
西北农林科技大学拔尖人才支持计划项目
关键词
小麦
MTDNA
RAPD
克隆
测序
Wheat（triticum aestivum）
mtDNA
RAPD
Clone
Sequencing
