白黎芦醇联合TRAIL基因对胃癌SGC-7901细胞增殖与抑制作用的实验研究

Experimental study on the effects and mechanism of resveratrol combined with TRAIL in inducing apoptosis and inhibiting proliferation of SGC-7901 cells

目的:探讨白黎芦醉(Resveratrol,Res)联合肿瘤坏死因子相关的凋亡诱导配体(Tumor necrosis factor-related apoptosis inducing ligand,TRAIL)在体外对胃癌SGC-7901细胞增殖和凋亡作用及其机制,为将Res作为抗癌药物的临床应用提供理论和实验依据。方法:以人胃癌SGC-7901细胞株为研究对象,将Res与转染了pBUD-TRAIL质粒的胃癌SGC-7901细胞共同培养,以MTT法检测细胞增殖抑制率;以FCM检测细胞凋亡率;以TUNEL法检测细胞凋亡等,观察联合应用对肿瘤细胞的抑制作用。结果:MTT和FCM检测结果显示:TRAIL组肿瘤细胞抑制率为54.28%,凋亡率17.12%,Res联合TRAIL组抑制率和凋亡率均明显提高,分别为70%和22.03%,与对照组相比,具有显著性差异;TUNEL检测结果显示:TRAIL与Res联合组与对照组相比较,细胞核明显棕黄色深染,且阳性染色细胞数目明显增多,表明TRAIL与Res联合作用凋亡细胞明显增加。结论:Res联合TRAIL基因可显著抑制肿瘤细胞的增殖和促进肿瘤细胞的凋亡,说明Res可增强TRAIL促进肿瘤细胞凋亡,两者具有协同作用。

Objective：To investigate the effects of resveratrol （Res） combined with TRAIL on gastric cancer and understand its mechanism,in order to lay experimental and theoretical foundation for clinical application of Res tumortherapy. Methods：SGC-7901 cells transfected with pBUD-TRAIL were processed with a 1/2 IC 50 concentration of Res. The inhibition of cell proliferation were examined by methyhhiazdyl tetrazolium（MTT）method,and cell apoptosis were detect by TUNEL method and flow cytometry using PI staining analysis. The findings in SGC-7901 cells transfected with pBUD and those in SGC-7901 cells transfected with pBUD-TRAIL were compared. Results：Compared with control group,the inhibition rate of the proliferation and apoptosis rate of SGC7901 cells in Res combined with TRAIL group were higher （70% vs 54.28%,22.03% vs 17.12%,respectively）by MTT and FCM. Karyopycnosis,nuclear chromosomal condensation and segmentation were observed by TUNEL. Res could significantly induce the apoptosis of tumor combined with TRAIL. Conclusion：Res combined with TRAIL could inhibit the proliferation and induce apoptosis of SGC7901 cell,demonstrating that Res could intensify the effect of TRAIL in promoting tumor cell apoptosis and have a synergistic action with TRAIL.