围生期双酚A暴露对雄性子代大鼠海马CA1区神经元形态及突触可塑性的影响

Effects of perinatal exposure to bisphenol A on the neuronal morphology and synapse plasticity in hippocampus CA1 of male offspring in rats

目的研究围生期双酚A(BPA)暴露对♂子代大鼠海马CA1区神经元形态及突触可塑性的影响。方法对SD大鼠的母鼠从妊娠d11直至产后7d每日分别皮下注射3种剂量的BPA(10、100、1000μg·kg-1作为低、中、高剂量组),同时注射食用色拉油设立对照组。统计各组母鼠产仔数,并观察母鼠吃仔情况。在♂子鼠出生21d后,取脑组织切片,进行HE染色;或用电生理学方法检测BPA暴露对海马CA1区LTP及LTD诱导率的影响。结果高剂量BPA组母鼠吃仔率明显高于对照组(P<0.01);HE染色结果显示,高剂量BPA组♂子鼠海马CA1区锥体细胞发生核固缩变性,异常锥体神经元数量明显增多(P<0.05);低剂量BPA组♂子鼠海马脑片CA1区LTP诱导成功率与对照组相比明显降低(P<0.05),而LTD诱导成功率则明显升高(P<0.05)。结论围生期BPA暴露可以损伤♂子代大鼠海马CA1区神经元形态及突触传递可塑性。

Aim To investigate the effects of bisphenol A （BPA） exposure to perinatal rats on neuronal mor-phology and synapse plasticity in hippocampus CA1 area of male offspring.Methods From the 11th gestation day（GD11） to the 7th postnatal day（PND7）,pregnant rats were subcutaneously injected with 3 doses of BPA （10,100,1 000μg·kg-1 per day as low,middle,and high dosage group） respectively,and with cooking oil alone as control group.The number of neonatal rats and the phenomenon of eating offspring in each group were recorded.The brains of male offsprings （after the PND21） were cut in slices and collected for HE staining or extracellular recording to detect rates of longterm potentiation （LTP） and long-term depressin （LTD）.Results The rate of eating neonatal rats in high-dosage group was significantly higher than that in control group （P0.01）.The HE staining results showed that the male offsprings in high-dosage group have karyopyknosis in pyramidal cells of hippocampus CA1 area,and more abnormal pyramidal cells than that in control group （P0.05）.The male offsprings in low-dosage group showed lower inducing rate of LTP（P0.05）,but higher inducing rate of LTD（P0.05） in hippocampus CA1 area than that in control group.Conclusions Perinatal BPA exposure may have adverse effect on neuronal morphology and synapse plasticity in hippocampus CA1 area of male offspring in rats.