UPLC法测定大鼠血浆中大豆苷元浓度及药动学研究

Determination of daidzein and its pharmacokinetics in rat plasma by UPLC

目的建立测定大鼠血浆中大豆苷元浓度的UPLC法,探讨大豆苷元在大鼠体内的药代动力学过程。方法 SD大鼠6只,ig给予30mg·kg-1大豆苷元混悬液,血浆样品经β-葡萄糖醛酸苷酶水解后,采用UPLC法测定大豆苷元浓度,并用DAS软件拟合并计算其药代动力学参数。结果大豆苷元的血药浓度在20μg·L-1～800μg·L-1范围内线性良好,提取回收率均大于85%,日间和日内RSD小于10%,符合生物样品分析要求。大鼠灌胃给药后,血浆中大豆苷元的药时曲线呈二室开放模型,主要药动学参数Tmax,Cmax,T12β,AUC(0-t),AUC(0-∞),CL,Vd分别为33.3min,355.4μg·L-1,915.7min,213.2mg·min·L-1,218.2mg·min·L-1,0.1467L·min-1·kg-1,74.4L·kg-1。结论该方法操作简便、快速、专属性强,可用于大豆苷元体内大批量样品定量分析及药代动力学研究。

Aim To establish a simple assay method for daidzein by ultra performance liquid chromatography （UPLC） and study the pharmacokinetics of daidzein in rats.Methods 6 rats were given 30mg·kg-1 suspension of daidzein via ig,the plasma samples were treated by enzyme,drug plasma concentration was determined by UPLC,and pharmacokinetic parameters were evaluated by DAS Software.Results Calibration curve was linear between 20μg·L-1～800μg·L-1.The recoveries of daidzein from plasma were more than 85%,and the relative standard deviations of the intra-day and inter-day were less than 10%.After ig administration of 30mg·kg-1 daidzein,the concentration-timecurves of daidzein were best fitted to a two compartment open model.The main pharmacokinetic parameters of Tmax,Cmax,T 21 β,AUC（0-t）,AUC（0-∞）,CL,Vd were 33.3min,355.4μg·L-1,915.7min,213.2mg·min·L-1,218.2mg·min·L-1,0.1467L·min-1·kg-1,74.4L·kg-1,respectively.Conclusion The method is simple,rapid and accurate,which is suitable for determination of daidzein in plasma and its pharmacokinetic study.