周围神经损伤后选择性再生的实验动物模型研究

Establishment of a Modified Selective Regeneration Model on Rats after Peripheral Nerve Injury

目的:建立并评价周围神经损伤后神经选择性再生的实验动物模型。方法:3周龄SD大鼠12只随机分成4周组和8周组各6只,均行切断右侧L2～L5背根神经节后纤维和右侧股神经横切-吻合手术,分别于术后4周和8周进行荧光逆向示踪,观察脊髓运动神经元再生情况。结果:4周组大鼠长入运动神经、感觉神经和长入2种神经的运动神经元数量分别为(167±21)个、(53±14)个、(36±7)个;8周组分别为(252±14)个、(37±8)个、(21±4)个。结论:该实验动物模型能反映周围神经损伤后运动神经再生的倾向性,重复性好。

Objective： To establish a rat modified selective regeneration model after periph- eral nerve injury. Methods： Twelve 3-week-old SD rats were randomly divided into 4-week group （n=6） and 8-week group （n=6）. The postganglionie fibers of L2-L5 dorsal root ganglia on the right side were cut off and the right femoral nerve was transectioned and then sutured in all rats. At the 4th week （4 week group） or 8th week （8-week group） after operation, the retrograde tracers （fluorescein dextran and tetramethylrhodamine dextran） were used to investigate the re- generation of the motor neurons in the spinal cord after injury. Results： In 4-week group, the numbers of neurons regenerated into the motor and sensory nerves and the both were （167±21）, （53±14） and （36±7） respectively. In S-week group the numbers were （252±14）, （37±8） and （21±4.4） respectively. Conclusion： It is an ideal rat model with good repeatability to study seleetive regeneration after peripheral nerve injury.