CAG方案对U937细胞株作用的体外研究

In vitro investigation of effect of CAG regimen on U937 cell line

目的研究CAG方案,即重组人粒细胞集落刺激因子(rhG-CSF)+阿糖胞苷(Ara-C)+阿柔比星(ACR)对U937细胞株的作用。方法以rhG-CSF(50ng/mL)、Ara-C(10nmol/L)和ACR(10nmol/L)单用或联用分别处理白血病细胞株U937,分别设为对照组(未加任何药物)、Ara-C组、rhG-CSF组、ACR组、rhG-CSF+ACR组、Ara-C+ACR组、Ara-C+rhG-CSF组及Ara-C+rhG-CSF+ACR(CAG)组。采用细胞计数仪计数并计算各组细胞的生长抑制率;流式细胞仪检测细胞表面分化抗原CD11B表达、细胞凋亡率及细胞周期的改变情况。结果在含Ara-C各组(Ara-C组、Ara-C+ACR组、Ara-C+rhG-CSF组和CAG组),加药后第1天,S期细胞百分比低于对照组。加药后第2天,细胞生长受到明显抑制,CD11B表达显著上调,其中CAG组显著高于Ara-C组(P<0.05);加药后第6天,细胞形态呈现部分分化,CAG组细胞分化特征尤为明显。各组细胞凋亡率比较差异均无统计学意义(P>0.05)。结论 CAG方案可诱导U937细胞分化,而且随着加药时间的延长,细胞的分化更加明显。

Objective To investigate the effect of CAG regimen of recombinant human granulocyte colony stimulating factor （rhG-CSF）＋ cytarabine （Ara-C）＋aclarubicin（ACR）on U937 cell line.Methods Leukemia cell line U937 was treated with rhG-CSF （50 ng/mL）,Ara-C （10 nmol/L） and ACR （10 nmol/L） alone or jointly,and control group （without treatment by any of the drugs）,Ara-C group,rhG-CSF group,ACR group,rhG-CSF＋ACR group,Ara-C＋ACR group,Ara-C＋rhG-CSF group and Ara-C＋rhG-CSF＋ACR（CAG）group were established,respectively.Cell proliferation inhibition rates were calculated by cell count analyzer,and the expression of cell surface differentiation antigen CD11B,cell apoptosis rates and changes of cell cycle were determined by flow cytometry.Results In Ara-C group,Ara-C＋ACR group,Ara-C＋rhG-CSF group and CAG group,the percentages of cells in S phase were lower than that in control group 1 d after treatment.Two days after treatment,cell proliferation was significantly inhibited,the expression of CD11B significantly increased,and the expression of CD11B in CAG group was significantly higher than that in Ara-C group （P0.05）.Partial differentiation of cells was observed 6 d after treatment,with significant cell differentiation characteristics in CAG group.There was no significant difference in cell apoptosis rate among each group （P0.05）.Conclusion CAG regimen could induce differentiation of U937 cells in a time-dependent manner.