日本血吸虫重组表膜蛋白Tetraspanin 2免疫诊断价值初探

Immunodlagnostic value of recombinant membrane protein Tetraspanin 2 of Schistosoma japonicum

目的 探讨日本血吸虫重组表膜蛋白Tetraspanin 2（rSjTsp2）作为免疫诊断抗原的潜能.方法 利用表达纯化的rSjTsp2建立间接ELISA法（rSjTsp2-ELISA）检测不同感染时期的感染日本血吸虫家兔血清特异性IgG抗体,并与成虫粗抗原（AWA）-ELISA法进行比较.结果 rSjTsp2-ELISA对感染日本血吸虫2、4、6周家兔血清特异性IgG抗体检测的敏感性分别为75.4%（46/61）、77.6%（45/58）和81.5%（44/54）,AWA-ELISA法敏感性分别为68.9%（42/61）、86.2%（50/58）和88.9%（48/54）,同一时期两种方法检测抗体的效果比较,差异无统计学意义（x2值分别为0.652、1.454、1.174,P均＞0.05）.结论体外成功表达了日本血吸虫表膜蛋白rSjTsp2,其原核表达重组蛋白具有一定的诊断应用潜能.

Objective To study the immunodiagnostic potential of recombinant membrane protein Tetraspanin 2 of Schistosoma japonicum（rSjTsp2） as a diagnostic antigen. Methods The rSjTsp2 was expressed and purified as an antigen used in indirect ELISA to detect specific serum antibody against Schistosoma japonicum of rabbits that at different stages of infection, and the results were compared with those of ELISA using natural antigen （AWA） as antigen. Results The sensitivities of rSjTsp2-ELISA were 75.4% （46/61）,77.6% （45/58） and 81.5% （44/54）, respectively, 2, 4, 6 weeks after rabbits were infected with Schistosoma japonicum, meanwhile the sensitivities of AWA-ELISA were 68.9% （42/61）,86.2% （50/58） and 88.9% （48/54）, respectively. The sensitivity difference of two methods was not significant statistically （x2 = 0.652, 1.454,1.174, all P 〉 0.05）.Conclusions The rSjTsp2 is expressed successfully in vitro, and rSjTsp2 recombinant protein expressed in prokaryotic system has a certain application potential in immunodiagnosis.