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大鼠角膜碱烧伤后角膜淋巴管生成及VEGF-C和VEG-FR-3的表达 被引量：2

Expression of VEGF-C,VEGFR-3 and lymphangion genesis in rat cornea after alkali burn
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摘要 目的:研究大鼠角膜碱烧伤后角膜淋巴管生成及VEGF-C和VEGFR-3的表达。方法:制作角膜碱烧伤模型,应用免疫组化方法检测正常角膜及烧伤后1,3,5,7,14,21,28d角膜组织中VEGF-C和VEGFR-3的表达,免疫组化LYVE-1(淋巴管内皮特异标记物)染色观察角膜中淋巴管生成情况,电镜观察烧伤后14d角膜新生淋巴管状况。结果:角膜碱烧伤后,VEGF-C和VEGFR-3的表达明显升高,烧伤后3d,角膜中出现淋巴管。结论:角膜碱烧伤后新生淋巴管生成伴VEGF-C和VEGFR-3高表达。 AIM ： To investigate the expression of VEGF-C, VEGFR-3 and lymphangion genesis in rat cornea after alkali burn. METHODS： The rat alkali burnt corneal model were made. The expression of VEGF-C, VEGFR-3 and lymphangion genesis labeled with LYVE-1 in rat cornea were detected by immunohistochemical assay at day 0 and day 1,3,5,7,14,21,28 after burn. In addition, the ultrastructure of lymphangion genesis in rat cornea was observed by electron microscope. RESULTS： The expression of VEGF-C and VEGFR-3 in rat cornea were increased after alkali burn. LYVE-1 were detected in rat cornea 3 days after alkali burn. CONCLUSION： There are some new lymphangion genesis in rat cornea after alkali burn. And meanwhile, the expression of VEGF-C and VEGFR-3 in rat cornea was significantly increased.
作者 郑彩慧 朱学军 张声 周琳瑛 徐国兴
机构地区 福建医科大学附属漳州市医院漳州眼科中心 福建医科大学附属第一医院眼科 福建医科大学附属第一医院病理科 福建医科大学电镜教研室
出处 《国际眼科杂志》 CAS 2010年第12期2246-2249,共4页 International Eye Science
关键词 淋巴管 淋巴管内皮细胞透明质酸受体-1 血管内皮生长因子受体3 血管内皮生长因子C 角膜 碱烧伤 大鼠 lymphatic vessels LYVE-1 VEGFR-3 VEGF-C cornea alkali burn rat
分类号 R772.21 [医药卫生—眼科]
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参考文献7

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共引文献18

同被引文献27

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引证文献2

国际眼科杂志
2010年 第12期

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