NaCl胁迫对6种红花幼苗渗透调节物质及抗氧化系统的影响

Effect of NaCl Stress on the Antioxidative System and Osmotic Regulation Substance of Six Varieties of Safflower Seedlings

采用沙培法,对红花(Carthamus tinctorius L.)6个品种幼苗进行不同浓度的NaCl胁迫处理,研究其叶绿素(Chl)、丙二醛(MDA)、可溶性糖(SS)含量及抗氧化酶(SOD、POD和CAT)活性的变化,并用隶属函数法对其耐盐性进行综合评定。结果表明:(1)随着盐浓度的增加,除封丘红花幼苗叶绿素含量逐渐下降之外,其它品种均呈先升高后降低的趋势;(2)盐胁迫下各种红花幼苗丙二醛和可溶性糖含量较对照均有显著增加,可溶性蛋白含量先增加后减少;(3)菏泽红花和川红花SOD活性在盐浓度为50mmol/L时与对照相比升高,随后下降,其它品种均呈下降趋势;NaCl胁迫下,6个红花品种POD和CAT活性都是先升高后降低;(4)应用隶属函数法,得出耐盐性的大小顺序为:封丘红花>菏泽红花>川红花>亳州红花>云红3号>新疆红花。

Changes in the amount of chlorophyll（Chl）,malondialdehyde（MDA）,and soluble sugar（SS）,and the activity of three antioxidant enzymes（SOD,POD,and CAT）were investigated after treatment with different NaCl concentrations.Salt tolerance was evaluated by fuzzy subordination methods.The results showed that with increasing salt concentration：（1）The total chlorophyll content of the Fengqiu safflower decreased gradually,while for the other safflowers,the content increased initially and then decreased.（2）The content of malondialdehyde（MDA）and soluble sugar（SS）enhanced significantly,and the soluble protein content initially increased,and then decreased.（3）The superoxide dismutase（SOD）activity of Heze safflower and Chuan safflower were significantly higher at 50 mmol/L salt concentration than that of the control,after which they declined.The others showed a constant decrease.With salt concentration increasing,the peroxidase（POD）and catalase（CAT）activity of all species initially increased and then decreased.（4）Using the fuzzy subordination method,the salt resis-tances of the six species decreased in the order of Fengqiu safflowerHeze safflowerChuan safflowerBozhou safflowerYunhong No.3Xinjiang safflower.