强直性脊柱炎患者外周血CD4^+ CD25^+ high调节性T细胞的表达及其与传统指标相关性研究

Study the Expression of CD4^+ CD25^+ high Regulatory T Cells in the Perpipheral Blood of AS Patients and its Corrlation with Traditional Marks.

目的研究强直性脊柱炎(AS)患者外周血CD4+ CD25+ high调节性T细胞(regulatory cells,Treg)表达特点,探讨CD4+ CD25+ high调节性T细胞在AS发生发展中的作用。方法采用流式细胞术检测30例AS和20例健康人外周血CD4+ CD25+ high调节性T细胞及HLA-B27,同时检测与AS相关的传统指标血沉(ESR)、C-反应蛋白(CRP)等,并分析CD4+ CD25+ high调节性T细胞与AS活动性指标的相关性。结果与正常对照组相比,AS患者外周血CD4+ CD25+ high调节性T细胞表达水平及CD4+ CD25+ high/CD4+比率显著升高(P<0.05),AS患者外周血CD4+ CD25+ high调节性T细胞表达与HLA-B27平均荧光强度呈正相关(r=0.623,P=0.027),而与HLA-B27的百分数及ESR、CRP无明显相关性(P>0.05)。结论 AS患者外周血CD4+ CD25+ high调节性T细胞表达数量增加,但其功能有可能是存在异常,AS患者体内存在免疫功能紊乱。

Objective To study the expression of CD4^＋ CD25^＋ high T cells in the peripheral blood of patients with ankylosing spon- dylitis （AS） and discuss its role in the development of AS. Methods Thirty AS patients and twenty healthy controls were enrolled in the study. CD4^＋ CD25^＋ high T cells and HLA - B27 in their peripheral blood were analyzed by flow cytometry. Le,Jels of ESR and CRP were also measured. Rusults Compared to healthy controls,the level of CD4^＋ CD25^＋ high regulatory T cells and the rate of CD4 ^＋ CD25^＋ high/ CD4^＋ in the perpipheral blood of AS patients were increased （P 〈 0. 05）. There was positive correlation between the expression of CD4^＋ CD25^＋ high regulatory T ceils and the mean fluorescent intension of HLA - B27 in the perpipheral blood of AS patients （ r = 0. 623, P = 0. 027） ,however, no correlation with the percentage of HLA - B27, ESR and CRP （ P 〉 0.05 ）. Conclusion The level of CD4^＋ CD25^＋ high regulatory T cells is increased in the perpipheral blood of AS patients, but its function is in lack. There is dysfunction with AS patients.